Vitamin D, 25-Hydroxy

Vitamin D, 25-Hydroxy

CPT Code(s):

  • 82306

Preferred Specimen:

1 mL serum

Minimum Volume:

0.5 mL

Special Instructions:

Collect blood in a standard red-top serum Vacutainer¨ tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately. Alternatively, collect blood in an SST¨, allow to clot at room temperature, centrifuge and remove from the gel within 24 hours.

Patient Preparation:

Fasting preferred, but not required

Transport Container:

Plastic screw-cap vial

Transport Temperature Name:


Specimen Stability Components:

  • Refrigerated: 7 days

Testing Schedule:




Reference Range:

<20 ng/mL Ð Deficient20 Ð 29 ng/mL Ð Insufficient30 Ð 100 ng/mL Ð Sufficient>100 ng/mL Ð Excess

Reject Criteria:

Gross hemolysis ¥ Gross lipemia ¥ Plasma ¥ Unseparated serum and serum in SST¨ not separated from the gel within 24 hours

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